Transfection efficiency

imagej

#1

Hi Guys,

I have transfected HEK cells with GFP and would like to estimate the transfection efficiency. I have worked out a Macro for counting DAPI positive cells but I am struggling with counting GFP positive cells. Can someone please help me? I have uploaded sample images (DAPI and GFP images) for help.
Thank you

DApiGFP


#2

Dear @Spandan_Kalra,

Great that you have worked that part out! Did you also take a look at http://imagej.net/Segmentation? You will find useful information on that Wiki page about how to transfer your selections.

Also, it would be very helpful if you could elaborate on what you have tried to do up until now!

Best,
Stefan