Transfection efficiency- counting cells



Hi all,

I’ve transfected cells with a Tye3 labelled siRNA and want to determine how many cells were successfully concentrated (transfection efficiency) from an image of these cells. How would you recommend doing this. I’m finding threshold adjustment for particle analysis difficult as i have grey cells on a grey background.




As you know,
In this image, it seems difficult to identify the edge of the cell.
So, I suggest taking a new image for this analysis.
How about using the marker which can detect the cell membrane or cytoplasm together with this Tye 3 labelled siRNA.