looking for some help here and I guess this is the best place.
I successfully managed to register a set of images in TrakEM2 with both automatic and manual corrections: really nice piece of software.
Problem is that I am interested in counting the average/sum of the pixel values in a ROI/AreaList and not just its area/perimeter/volume. Do you know if there is any chance to do this? Whenever I launch a measurement there is no information on the pixel count.
Alternatively I tried to export my grayscale images and perform this analysis with ImageJ afterwards but the only way to export figures is 8 bit while I have a 16 bit and then I lose the information that I have in the pixel values.
If anyone has a solution I would love if he or she could share it.