I’m totally new both to this forum and to Trainable Weka Segmentation plugin; as far as I could see, my question hasn’t been answered previously (although this error was mentioned in one post); if it has, I apologize.
So, I’m trying to get to know the plugin by putting in a time lapse image stack of a few cells overexpressing RFP or FRET construct. I obviously want to segment the images into individual cells for further analysis (they change morphology over time). Yet, I can’t even start, I just keep getting the message “Cannot train without at least 2 sets of examples!”, and accordingly, “The training did not finish”.
It must be something very simple that I’m doing wrong?
I apologize again if this has been discussed before.
Many thanks in advance to anyone who can help!