I’m having trouble with the TrackObjects module- while it seems to be tracking and naming the objects correctly when I look at the labeled images (frames 1&2 from a sample movie attached- notice that for example 3&5 are conserved from frame 1 to frame 2 and the highest visible number (79) is the same as the number of objects), when I look at the .mat and .csv outputs those numbers don’t match up at all with the image outputs (for example, the highest value is 52) and multiple objects are given the same label!
I’ve attached the pipeline, the .mat output, and the first 2 frames of the TrackObjects graphic output- the original movie is too large to attach(61Mb@16 bit) so I’ve given you the first 10 frames@8bit. Thanks so much!
DefaultOUT__1.mat (1.57 MB)
_pseudofinal_ColocalizationTelomereTracking.cp (14.7 KB)