TrackMate velocity unit

I used trackmate to generate velocity color-coded tracks. Now that i’m writing figure legends, I’m not sure what the units are for the velocity spectrum. Was it calculated based on my parameters? I remember specifying the um/pixel for my data, but not the time between each frame of my video. So perhaps the velocity was calculated as um/frame? Or are the units arbitrary?

Many thanks for answering my question!

No you are correct. TrackMate uses whatever units you put in the image metadata.
So if you have um in the spatial calibration and frames in the time calibration, the velocity units will be um/frame.

More info here:
TrackMate Algorithms - ImageJ.

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