Same as trackmate-tracking-at-high-speed i have some issues to track High Speed particles with Trackmate.
In my analysis the particles have very straight movements with roughly constant velocity vectors:
Substack (1-200).zip (9.8 MB)
Because of that i am using the Kalman Tracker (Linear Motion Tracker).
It works well, but not perfect.
Because of the High Speed, i need a large search radius. And this leads to the fact, that Trackmate detects Tracks between one particle and another particle, which is closer than the same particle a frame later. You can see the edges here:
or as .avi: TrackMate capture of Substack.zip (19.7 MB)
Did anybody have the same issue and how did you fix it?
If the Tracking Algorithm would have the information, that adding spots to the Track only makes sense if the new spot is in line (Trendline of the Track would intersect the new spot) to the Track.
Another way to do it would be with the angle from one spot to the following spot of a Track. If the angle is above a value the spot will not be added.
Is it possible to modify the Tracking Algorithm?