I have a confocal image, having 1 fluorescent channel,10 number of Z slices, and 10 number of time points or frames.
I have already selected ROIs at a particular Z slice using ROI manager. my question is,
How can I track the intensity of the ROI over the time points using TRACKMATE. The shape of ROI would drift in less than 5 microns at any two frames.
Is there any specific plugin available for this application?
any help would be appreciated.