I’m analyzing images acquired that are about flow in pipes containing markers, and I’m trying to track the markers using the Trackmate plugin.
The Trackmate is actually doing great when the flow speed is low, however, when the speed is too high the LAP tracker links the marker to the neighboring marker because it is closer than the same marker spot in the following frame!.
I’ve tried to modify the tracks manually using the TrackScheme, but with more than 5000 frames in each data set, it is a very difficult mission and I assume it is not the optimal way to do it.
I also think that assigning a specific trajectory for the tracking (along the y-axis of the pipe for example) or adding an angle parameter to the linking will be useful, instead of just depending on the distance to construct a link, but my coding knowledge is limited.
(I’m using The LoG detector and The LAP Tracker in the setup).
Attached you can find an image of a tracking done for just 200 frames, the color map is for Mean velocity, as you can see the markers in the middle of the pip[e are not well tracked due to high speed, however, the markers on the side of the pipe are better tracked due to a lower speed.
I need advise on this problem, please.
Thank you very much for helping.