I’m currently working on mitochondria transport with Trackmate (and Track Analysis). I am desperate to quantify the amount of anterograde and retrograde transport of mitochondria along axons. I can not find this information with Trackmate (neither Track Analysis). I have reoriented and stabilized all my films, movements are therefore predominantly in X. So here are some questions :
1-Is it possible with Trackmate to have, the Delta Xt0-Xtfinal?
2-To determine the time spent making a retrograde or anterograde move during the recording? (like what can be done with manual tracking for example)
3-To have the relative speed? (minus 0 : retrograde and plus 0 : anterograde) Maybe by adding a reference point?