I am working on measuring mean intensity of vesicles from each track. I am aware that Trackmate calculates mean intensity of each track using physical radius, R. But I want to estimate mean intensity based on estimated diameter which is calculated from contrast difference. To do this, I am now trying to write a script in MATLAB or Python. I was wondering if I can edit the Trackmate script to do this task or any other API would do. Any comments or suggestions would be helpful!
Thanks for your time! I am really grateful for your quick responses in my past queries!