In our group we are interested in endocytosis, so we track endocytic invaginations following two different fluorophores (via Optosplit) and measure the intensity change over time. For this purpose, we are using Manual tracking of TrackMate: we add spots of a particular size, track them manually, and then measure the intensity.
However, we would like to know if there is any tool to copy the spots/track created in one stack (fluorophore 1) and paste them in another stack (fluorophore 2). We explored the option “copy overlay to” but it did not work for more than a single frame of the stack.
Thank you very much for any help