My name is Hiteshika Gosain and I’m doing my PhD from WWU Münster, Germany. The topic of my thesis is “Super-resolution analysis of the membrane domain formation during the neuronal Polarisation”.
We are focusing on the exocyst subunits and want to Analyse the spatial and temporal resolution of all these subunits. To do this we are using single-particle tracking using TIRF.
I was using the trackmate and @msdanalyser program designed by you, and I must thank you for writing such a wonderful program.
If you have some time, I would like to bother you with some questions.
By far, I have been using a single colour for tracking purposes but now I want to extend my research to analyse 2 colours/multicolour for co-localization and co-tracking.
This may help me to understand where in space and time do these subunits assemble near the plasma membrane.
Can you please guide me if there’s a method to do multicolour tracking for two different protein of interests in the trackmate or have you designed another program for the same? If I split the channels, and then evaluate the single channel, is there a possible way to combine them later to get co-tracks?
Is there a way to determine the cluster analysis in order to check how many protei of interests constitute a single bright spot? Can we do this by Spot analysis in the trackmate?