TrackMate - "Estimated Diameter" output

fiji

#1

Hello everybody,

I use the TrackMate Plugin to perform a single particle analysis on lysosomes; moreover I need to correlate dynamic information and size (diameter) of the organelle. The TrackaMate output “Estimated Diameter” gives back a distribution which appears to be not continuous, as it should be, but “quantized” on fixed values (see attached file). This could bias the diameter estimation in the spots statistics results. Is it possible to fix it?

Thanks!

TrackMate_EstiDiam_T


#2

Hi @William

Unfortunately the Estimated diameter feature computer is very crude. It is not robust against noisy images and objects that are not spherical.

I unfortunately have not a better solution right now, unless someone else made a TrackMate module that address that. People?