Trackmate autotracker not recognizing my particles

hey,
I am trying to use trackmate plugin in order to to see what is the trajectory of my planulea (a cnidarian larvae).Unfortunately in the stage where the program supposed to recognize my planulae (seen as black spots on a white background) it actually recognize a bunch of spots that are not my planulae. I tried to use all types of detectors with different parameters but still not success. Can anyone know what am I doing wrong or how u can fix this problem.

thanks!!
Menahem

Hello @Menahem

TrackMate tracks bright spots over black background.
Try to invert yoiur image first (Shift-I)

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