I made a short pipeline for cell (nuclei) tracking - LoadImage, IdentifyPrimaryObjects, TrackObjects, SaveImages, ExportToSpreadsheet.
I tried the LAP tracking and in principle this pipeline functions but I encountered a strange problem. The tracking module “connects” correctly allways two subsequent images but for the next two images it completely renews the IDs of objects. I tried to change the parameters of the tracking and it basically does the same mistake. Am I missing something? What can influence this? It seems quite strange because in one step it tracks (connects) the objects fine but in the next it fails. I am almost sure it has a simple solution But I am not experienced enough to solve it. I have binary mask images made from from low magification images, so its tens - hundreds of objects.