Posting for one of our students:
I have single molecule imaging data showing molecules on a membrane diffusing into and out of a region of interest. I am trying to make a figure and/or movie that highlights tracks that start outside the region and move in, or start inside and move out.
I have successfully tracked the molecules using TrackMate, but cannot figure out how to show all the tracks I am interested in simultaneously, i.e. tracks filtered by start/end point inside/outside ROI. Also, when I export an overlay of a single representative track, the line is too thin to be easily visible, and I can’t seem to change the line thickness.
Can anyone help me figure out how to solve these problems in Trackmate? Or can anyone suggest a tool that can take in my Trackmate XML data and output a movie or figure with the tracks of interest overlaid on the original images?