I am still fairly new to CellProfiler and would like to know more about analyzing migration trajectories and the change of location for each cell between frames. I have images for a 2 hour time-lapse where 20 images are taken at different locations which is at one frame and then the time-lapse starts over at the original frames to track the cells, which are shown in DAPI. I am also counting the cells that are in the tapered channels versus the cells in the loading channel on the sides. I have a general pipeline that I have created, but I do not know how to continue adding to the pipeline to achieve migration trajectories, the exact change of location for each cell between two frames, and measuring migration velocity. I have also tried downloading the example on the CellProfiler website for tracking cells, but even making modifications for my images it does not seem to work as I would like.
Any help would be great!
Here is the project including images:
1-18-17SqueezeAnalysis.cpproj (590.6 KB)