Tracking and measuring objects from a confocal temporal data

3d
confocal
identifyprimary
cellprofiler-analyst

#1

Hi,

We’re trying to read neural activation out of a 3D confocal temporal data.
For each time point we have a confocal z-stack of ~40 layers, in two different channels.

We would like to identify objects in one channel and to measure their intensity in the other.
I’ve been playing with some of the pipelines/modules and couldn’t quite understand if CellProcedure supports such 3D operations.

Is there any similar pipleine I can consult with ?

Thanks a lot in advance,
CellProfiler rocks.
Eyal.


#2

Hi @Eyal_Itskovits,

Have you seen the 3D tutorial? Might be a good starting point.

Thanks!