I have a series of images taken over time. Objects in the image may be swelling slightly over time. I’d like to track and quantify this swelling.
It seems like the TrackObjects module (cellprofiler.org/CPmanual/TrackObjects.html) would be useful, but from what I can see, object size is not one of the available measurements for the TrackObjects module.
Does anyone have any suggestions as to how to track the size (and total intensity, BTW) of individual objects in a series of images?