Hello to you all!
I am new here and I have had a quick look on the cell profiler website. However I cannot find anything related to cartilage/bone toluidine staining quantification pipelines.
Basically, I want to quantify the amount of toluidine blue positive regions out of a region of interest, however cartilage is purply stained while bony regions remain light blue.
Is there a way to quantify this and how do you do this?