This is an minimal working example that i found from cellprofiler blog
I’m pretty new to cellprofiler and still working my way around the software. I have come across some difficulties trying to do segmentation on Mouse spleen tissue stained with nuclei-DAPI and CD11b-AF488. I was hoping to find some answers/help here!!!
- Ideally, I would like to segment my tissue based on Nuclei and CD11b staining, by segmenting I mean to identify CD11b+ cells on nuclei.
Can anyone please help me build a pipeline that can achieve required analysis? Any kind of help would be greatly appreciated!!!