Tissue neighborgs

We are trying to highlight the cell borders in a tissue stained with ZO-1. We have managed to get the segmentation, however, there are some areas (more than one cell, without stain) that we will like remove from the calculation. We are absolutely beginners… Thanks a lot for any help! :smile:

Hi,

This looks mostly like a duplicate of this post, so we’ll just respond there.
Thanks,
David