Threshole correction factor

cellprofiler

#1

Dear all,

How to determine the specific threshold correction factor value? Why the value is 0.8 in the module IdentifyPrimaryObjects in the example
http://cellprofiler.org/examples/#TissueNeighbors

Thank you for any advice!


#2

Hi,

The threshold correction factor value is usually advised to tune last , after you test all the different combination of threshold strategy and method .

For e.g.:

Global > Otsu > Two classes
Global > Otsu > Three classes (middle class assigned to Foreground/Background)

Adaptive > Otsu > etc. etc…

Only whence you really need to perfect the object segmentation, you would need to change the correction factor. Leaving it at 1 would most often be more robustly applicable to different sets of images (i.e. not to “overfit” to just one image)

Bests


#3

Dear Minh,
Thank you very much for the reply!