Previously, when I would threshold one of my images with the dark background feature checked, the background would be white and the cells I was counting would be black and upon analyzing the particles I would get an accurate cell count. However now when I threshold doing the same thing, the background is black and the cells are white. When I change the thresholding bars to make the background white and cells black it just counts the background as one large cell and gives me a very low count of about 1 or 2.
Does anyone have any idea why this may be or how I can get my thresholding properties back to what they initially were? Any assistance would be appreciated!