I have some pictures to analyse with “analyze particles” because I want to measure the area, area fraction, counting and etc. My problem stars when I choose the threshold ( I tried to take a pictures the most black background and same light intensity), but the problem follows when I run analyze particles (some ROI selection include non-interested particles, so the background).
At the end I get:
- If I use “include holes” = a large ROI selection with some background places
If I don’t use “include holes” = a large ROI selection sometimes without background places, sometimes a large Selection and another small included selection.
I would like to have as result only one selection that takes the real particles and non the black background as another new included selection because this can be an error in measurements.
Thanks a lot for your help,