Hi @neel,

The skeleton of a binary object is a *centerline* (see here).

This means that skeleton of a binary object should (must) have exactly the same thickness everywhere, otherwise its not a line - lines have no “thickness”. This is why the mean and max are the same: because the thickness is the same everywhere.

**Because of this, I don’t think it’s meaningful to measure the thickness of a skeleton.**

That being said, I’m surprised you’re getting a value of 2. (I might expect 1, presuming the skeleton is measured on a pixel grid with a one-pixel “thick” mask. I’m not familiar with the BoneJ implementation, but it may be that your choice of connectivity rule could affect the value of thickness you get.

From here:

defines the thickness at a point as the diameter of the greatest sphere that fits within the structure and which contains the point.

Hope this helps, but post back if you have more questions,

John