In my study, some cells express GFP and others have no expression. I use CellProfiler to analysis the intensity of the GFP expression. First, I use the IdentifyPrimAutomatic Display to outline nuclei. Then, I used the IndentifySecondary, but I found the cells that haven’t expression has the nuclei’ outline that shouldn’t have in the picture. How can I do to analysis the cells that only have GFP expression?
Thanks for your help.