I would like to use the data exported by Kappa, and I have known that the measurements are based on a scale factor (in μm/pixel).
But does the curve length refer to the length in the picture? Does zooming in and out of the picture affect that length?
The picture I use is 500×333 pixel, does it means the curve length is 159.1544×500×333? Isn’t the value too large?
Any help would be greatly appreciated.