I just want to ask a simple question relating to my experiment. I firstly need to identify the cytoplasm of cell by using whole cell stain and DAPI and I have successfully done that by identify primary, secondary and tertiary objects. Then I need to calculate the intensity of cytoplasm of each cell. However, I only can generate the intensity value as a sum of all cytoplasm I outlined. Is there any module I can select to measure and report the intensity of every cell as a list. That will be really helpful because now I have to draw every single cell manually as region of interest on ImageJ which is killing me. I uploaded a picture which is the merge picture of the DAPI and my GFP labelled protein. I want to know the intensity of the cytoplasmic area of each cell, Mean value and the min and max. I have single channel images.
Also, I always get the intensity as a number between 0-1. is there any other range? like 0-4096. My image is 12bits.