Hi, I have struggled with a basic function of FIJI for several days. As described in the“Image J user guide”，when i do not choose “include holes”, the internal holes inside the particle can be recognized. Internal holes will be ignored only if “include holes” is chose.
But , whether I choose it or not, internal holes always were ignored. As show in the below image, same results was got. It confuses me.
Thanks very much if anyone can help.