I’m trying to use CellProfiler, to generate bands around nuclei, but I would need that this band are limited to the cell body (eg the band can not be out of the cell), and I have this two channels:
So far, I detected the nuclei (on channel 2), expanded the nuclei, created a Secondary using the nuclei and the red channel (cell). From these objects I created Tertiary objects, using either “cell and nuclei” or “cell and expand nuclei”. When I combine the 2 newly created object I have an unexpected results, some weirds outlines remaining, cf image below :
I thought a workaround would be to shrunk it , but it doesn’t work as I would expect:
I wonder :
- if my approach is over-complicating things (eg maybe there is a module that does extactly this but I didn’t find it yet)
- if what I get with the tertiary operation is expected or is it a bug ?
Thank you for your help,