We have build a T-SPIM but not sure if there is any way to operate with the FIJI plugin other than to have illumination from both directions simultaneously. Even if i could run a device that could shutter the laser to each side consecutively I don’t know how i could configure an acquisition in the SPIM plugin to image the channels on each side interleaved i.e. Z plane 1, left axis, channel 1, channel 2, Z plane 1, right axis, channel 1, channel 2, Z plane 2, left axis, channel 1, channel 2 etc etc or indeed how to do the z stack of one axis first then the second but this would not be the preferred approach. Is anyone doing this in Micro-Manager?
Thanks for any advice,