I have a few hundred of these cryo-em pictures of synthetic vesicles.
They are all roughly spherical and I just need to measure their diameters. The large structure that dissects the image into three parts is just the TEM grid and should be excluded from the analysis.
1.tif (1.0 MB)
I’m having issues detecting the edges of the vesicles since they are only a slightly darker grey and are only a few pixels wide.
I’m using Fiji but am new at the automation. Plugins like particle analyzer can’t seem to differentiate the vesicles from the background no matter how I set the threshold. I also tried diameterj, but it also can’t seem to detect the edges of the vesicles apart from background.
I also looked into trying to use cell profiler but don’t know where to start as far as the image processing side of things goes.
Thank you for any help!