I’m using MGX for my meristem confocal time-lapse to analyze changes in cell proliferation and expansion, but I’m having some troubles of making seeding the cells consistently and more efficiently. All the online tutorials I found are talking about seeding cells with alt-key and the “add new seeds” tool to outline each cell, but this became a very time-consuming process when each of my samples has about 500 cells, and I have multiple replicates per stage, and multiple stages. I’m also worried about the consistency issue in my own judgment in outlining the cells, since sitting in front of the screen to outline cells for an hour made my eyes fatigue and misjudge cells.
I’m wondering whether there’s a more efficient way of seeding the cells? Any comments and suggestions will be very appreciated!
Thank you in advance!