Hi, I am looking for suggestions about what tools might work best for this problem. I am trying to quantify as a percentage area covered the amount of tissue occupied by the lipid based vaculoles (While circular objects) in this image that are not the lumen (Inside hole) of the tubules represented.
I think my approach should be to isolate (Segment) the tissue area, for instance not all of my Images will be entirely tissue see upper left corner for example. then segment out the vacuoles but I’m not finding tools that preform this well.
Does anyone have suggestion regarding good tools or a better approach?