I have been trying to use the correct illumination calculate/apply modules but have not had any luck. I have tried a number of things and looked at the tutorial but have had little success. I was hoping I could get some input on setting the parameters. I have different time series with 6 time points, and each series will need a different correction. I’ve attached an example time lapse below. You will see the circular artifacts scattered throughout the images that persist throughout the time lapse. They have been causing some issues during segmentation so I’d like to remove them if possible!
I look forward to your feedback!