I am just acquainting myself with CellProfiler (great program!) and have a question about the settings for the subtract module. I would like to use an image as a negative control and subtract the average intensity of that image from the intensities of subsequent images. Ultimately, I would like to generate intensity histograms for images that have been corrected in this manner. I see that there is a subtract module to perform this task but am having trouble instructing the module to make this adjustment – I assume I’m not providing the right commands. I appreciate your help on this matter.