I am struggling trying to align several images using ImageJ. The images are the following:
- 4 images of the same spot in the tissue that belong to 4 different channels (blue, yellow, magenta and green)
- 2 images of the same spot but at different time point. In this case the staining for yellow is the same but green is different (magenta and blue are missing).
I would like to align these 6 images together based on the alignment of the yellow channels since they are the same. The rest of the channels have different staining therefore I cannot align all the 6 images together (or at least I have not been able to do so).
Can someone give me an approach to accomplish this task? Sorry for this potential naive question.