I’m using the straight line tool to measure fluorescence intensity along the line in two channels.
Therefore I use the Plot Profile and I’m interested in the distance [μm] (between two intensity maxima along the line). Plot Profile -> List shows x and y values.
My question is, is there a possibility to set the x values. Right now the Plot Profile shows the x- values 0.000 μm, 0.0648 μm, 0.129 μm, etc. Can I change these values (reduce the interval)? Or is there a possibility to measure the distance [μm] of two intensity maxima of two channels along a straight line?