while MIB offers so many fantastic tools for image alignment, I was wondering if there was an option to stitch multiple stacks of multi-ROI image acquisitions with serial EM data. We were imaging a blood vessel, that kept on “dividing” or rather it had to be imaged with several ROIs, before ultimately converging into one.
The problem is, that we did not know where the vessel would move to, so we were adjusting the FOV window a lot and not at all times, the ROIs do overlap, in order to keep imaging time as short as possible. Now all ROIs have a big canvas size with lots of empty space after alignment. Additionally, we were adding and removing ROIs when the vessel converged or split up. This is giving me a hard time with Big Stitcher and Fiji alternatives at least.
Are there any existing features that I miss, or any plans to add any in that regard? Maybe you have also have a tip which program can help me with that. I currently do not have access to Amira unfortunately.
Keep up the great work,