Briefly, I’m trying to stitch a whole well of a 6 well dish in FIJI, taken with some overlap, then re-split it back out into larger, non-overlapping sections. All of this is done in a single script.
I’ve been working well with Grid/Collection stitching, but our collaborators recently increased the size of the field they want stitched and I’m now above the 2 gigapixel limit. I’m trying to use BigStitcher but without much success; I have 1364 tiles, but it then claims it can only recognize 1351 and when it loads them it says it’s got 1338 (not sure if whether the drop is symmetric (13 each time) is relevant or not). When I try to then fuse it anyway, it then complains I’m above the 2GP limit. If I try to split without fusing, it gives me a NullPointer exception.
Is there something else I can try? Will Grid/Collection make >2GP images if running headless?