Please help me, I have generated a set of confocal images (with each image having the file name format e.g. W0014F0001T0001Z000C1). I have 83 wells, 4 fields and 18 z slices in total. I want to produce a 3D image of the tissue slice based on these images however first need to organise the image sequence by merging the channels and stitching together the fields. Is there an easy way to do this?
Have you tested this ? https://imagej.net/Stitch_and_Align_a_sequence_of_grid_images_Tutorial