Starch quantification using stained image


I want to quantify starch in leaves of plant. I removed the chlorophyll to turn leaf white. After that it was stained in Lugol iodine solution for 5min. Destained/Rinsed in water and then photographed.

How can I quantify this stain (see blue color in image attached) using image J (OR) any other software that you can suggest?


I would point you to using the Trainable Weka Segmenation (TWS) plugin in Fiji. That would be a great place to start. You can search here on the forum for others that used this tool… there is also a video tutorial where I show the ‘basics’ in using this plugin (and here are the matching slides and also the updated slides).

One older example here on the forum (there are many others):

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