I’m back with a new question! after having set up a decent pipeline for the IHC staining (DAB) I’m trying to figure out how make some simple statistics.
I’m running more than one image of each groups and at the and I have the stdev of any single image. it is correct to make the mean of the stdev in order to have only one value to put in the graph?
I have attached the pipeline and the results…
I would say it’s correct if the underlying distribution supports it. If you think that the standard deviations collected represent samples from a normally distributed population (or approximately so), then yes, the mean would be appropriate.
Thanks a lot!