Stain vector for TMA analysis

Hello,

Do I need to estimate stain vector for each core I analysis? Or will it suffice to do the preprocessing once at the beginning?

This might be a trivial question but I could not find an answer after googling with many different keywords…

Thank you for your response in advance!!!

Ideally the stain vector is for the stain - and is obtained by a single stain sample. The tissue should not be relevant.