Spot number from the first frame is a bit less compared to the rest in TrackMate

Does anyone have observed that the spot detection in TrackMate always has a bit less spots in the first frame compared to the rest (around 5-10%)?

I didn’t observe anything like this. Can you share an example image stack to reproduce the issue?

Now I figured out why: the number for all spots is stable, but the number of spots in the track is less in the first frame!