Split .tiff into 4 channels

Hi, this might be a very naive question, but there a quick way to split .tiff confocal microscopy image into 4 channels? I am only managing to split it into the 3 (RGB). The fourth color is cyan and it blends with blue when split.

Thank you!

If your file has been saved as a colour RGB .tiff file, the cyan data will split between blue and green channels. You need the original confocal file or multichannel .tif file (i.e not overview) to split in different channels.

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