Spheroid Analysis

Hi All,

I was hoping to use CellProfiler to analyze cell migration in spheroid images but am not quite familiar enough with CP to know where to start. I would like to be able to measure a change in radius of a cell between two images. I found a forum post here that described an ImageJ tool that allowed for a plot of integrated intensity as a function of the radius that seemed like it would be helpful, but I’m not sure if CP offers anything similar or any other way to go about this.

Thanks for any help!

Nick

Hi,

You can try our example pipeline of tracking flying embryos. There are several tracking methods available in CP tracking module. You can try overlap/Distance method for tracking spheroid images.
ExampleTrackObjects.cppipe (13.1 KB)