Speckle identification

cellprofiler

#1

hello (sorry about the previous post)

I have been using the speckle pipeline module from your demo page to count the number of protein aggregates within a cell. Your module works very well with the attached image file. However, when i attempt to using the pipeline on my images it does not seem to recognise the nuclei and subsequently the rest of the program fails to work. Could you help me with this problem
The images are avaliable at the following link
[img=http://img2.freeimagehosting.net/uploads/th.9cc6ac27eb.jpg]

Thank you
shoey


#2

Hello,
I cannot view the images on the links that you posted. Please let me know if there is an alternative link that will work.
thanks,
martha


#3

How about these:

img2.freeimagehosting.net/image. … 4e5ee1.jpg

img2.freeimagehosting.net/image. … ac27eb.jpg

Thank you and best wishes
Shushant


#4

Well, it looks like the image for nuclear staining is out of focus. Additionally, it does not look like that stain is only staining the nucleus. If those are the case, it would be worth optimizing your protocol before trying to quantify the speckles.
~martha